Compound D presently only exists as a spray. difficile PCR ribotypes, representing an outbreak related PCR ribotype (027), an endemic PCR ribotype (014) and a non-toxigenic PCR ribotype (010).įull size table Disinfecting cleaning wipes and spraysĬompounds A, B and C were tested in the form of a wipe, as well as in the form of a spray. These products were tested for their efficacy against three different C. In the present study, four products were tested that are most commonly used as cleaning and disinfecting products in hospitals in the Netherlands. Therefore, alternative agents are needed to eradicate spores of C. However, hypochlorite has to be used in excessive concentrations to be effective, thereby increasing its toxic and corrosive properties. It is known that hypochlorite does not enhance sporulation and when used for environmental disinfection leads to a significant reduction of C. difficile, it is generally advised to use an unbuffered 1:10 dilution of hypochlorite. To disinfect environments contaminated with C. Guidelines to date only advise to take extra measures with CDI diagnosed patients, for example the guideline for disinfection and sterilization in healthcare facilities, 2008, of the Centers of Disease Control and Prevention (CDC). Still, the chance of transmission from asymptomatic carriers is probably lower than from patients with a CDI. difficile conclude that asymptomatic carriers can also introduce the bacteria into the hospital and may consequently play an important part in the transmission to other patients. difficile can survive in hospitals for years. The hospital environment is known to be a key pathway for patients to acquire C. Effective infection control measures are therefore greatly needed. difficile is estimated to be €4 billion for Europe, $1 billion in the United States of America and $280 million in Canada. The yearly national excess hospital cost associated with hospital-onset C. These costs arise due to direct healthcare costs and due to longer hospital stays. difficile are estimated between $8911 and $30,049 per case.

difficile is an important health threat associated with morbidity, mortality, and extra costs. difficile infections doubled between 20 in the United States of America. difficile is emerging worldwide in healthcare facilities. Wipes with hydrogen peroxide (1.5%) showed the highest bactericidal activity.Ĭurrently C. In general, impregnated cleaning/disinfection wipes performed better than ready-to-use sprays. difficile spores of PCR ribotypes 014 and 027 strains are more difficult to eradicate than non-toxigenic PCR ribotype 010. The wipes had a higher RLU log 10 reduction, but no significant difference for RLU reduction was observed between the different C.

On average, although not significantly, a difference in relative light units (RLU) reduction between the wipes and sprays was found. Overall, the wipes performed better than the sprays with the same active ingredient. difficile PCR ribotype 010 was highest, followed by the reduction of C. Irrespective of the disinfection method, the microbial count log 10 reduction of C. When products neutralized after 5 min, microbiological cultures and ATP measures were performed. The tiles were left to dry for an hour and then wiped or sprayed with one of the sprays or wipes as intended by the manufacturers.

difficile strains belonging to PCR ribotypes 010, 014 or 027. Tiles were contaminated with a test solution containing a concentration of 5x10 6CFU/ml spores of C. Methodsįour different products were tested 1) hydrogen peroxide 1.5% 2) glucoprotamin 1.5% 3) a mixture of ethanol, propane and N-alkyl amino propyl glycine and 4) a mixture of didecyldimonium chloride, benzalkonium chloride, polyaminopropyl, biguanide and dimenthicone as active ingredients. In this study different cleaning/disinfecting wipes and sprays were tested for their efficacy against spores of distinctive C. Appropriate sporicidal disinfectants are needed to prevent development of clusters and outbreaks. In healthcare facilities, Clostridium difficile infections spread by transmission of bacterial spores.

C diff cleaning wipes